Optimizing the use of Sinapis alba seed meal extracts as a source of thiocyanate (SCN−) for the lactoperoxidase system
| Autor: | C. I. Nindo, Inna Popova, Matthew J. Morra, Amir Golmohamadi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Meal biology Thiocyanate Sinapis Lactoperoxidase Substrate (chemistry) Hypothiocyanite 04 agricultural and veterinary sciences biology.organism_classification 040401 food science 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0404 agricultural biotechnology chemistry Biochemistry Generally recognized as safe biology.protein Glucose oxidase Food Science Nuclear chemistry |
| Zdroj: | LWT - Food Science and Technology. 72:416-422 |
| ISSN: | 0023-6438 |
| DOI: | 10.1016/j.lwt.2016.05.007 |
| Popis: | The multi-component lactoperoxidase system (LPSys) is used for improving food safety and requires thiocyanate (SCN − ) as a substrate for the generation of antimicrobial hypothiocyanite (OSCN − ). Production of OSCN − is usually limited by intrinsic concentrations of SCN − or else SCN − must be supplied from a synthetic source. Our objective was to optimize the production of OSCN − in the LPSys using SCN − derived from glucosinolates present in yellow mustard ( Sinapis alba ) seed meal. Thiocyanate was produced from S. alba seed meal by shaking a 1:20 (w:w) meal:H 2 O mixture at 70 °C for 10 min. Increasing the exogenous H 2 O 2 to an equimolar concentration (500 μM) of SCN − further increased OSCN − production, whereas H 2 O 2 concentrations in excess of SCN − enhanced OSCN − decay. Glucose and glucose oxidase (GOx) were substituted for H 2 O 2 to sustain OSCN − production resulting in an optimized LPSys containing 4.92 U/mL lactoperoxidase, 64 mM glucose, 342 U/L GOx, and 500 μM SCN − . Maximum OSCN − in the optimized system was 40 μM, a concentration that is predicted to have antimicrobial effects, thus demonstrating the potential of the low value S. alba seed meal as source of SCN − for preparing a generally recognized as safe LPSys. |
| Databáze: | OpenAIRE |
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