Inhibitory Effect of Green Tea Polyphenols on Membrane-Type 1 Matrix Metalloproteinase, MT1-MMP1)

Autor:	Shoji Yahara, Motomi Matsukawa, Tomohiro Asai, Naoto Oku, Fumio Hashimoto, Satoru Yamakawa, Toshifumi Akizawa
Rok vydání:	2003
Předmět:	Pharmacology chemistry.chemical_classification Matrix metalloproteinase inhibitor Angiogenesis Flavonoid Gelatin Zymography food and beverages Pharmaceutical Science Catechin General Medicine Matrix metalloproteinase Umbilical vein chemistry.chemical_compound Enzyme chemistry Biochemistry
Zdroj:	Biological and Pharmaceutical Bulletin. 26:1235-1238
ISSN:	1347-5215 0918-6158
DOI:	10.1248/bpb.26.1235
Popis:	Matrix metalloproteinases (MMPs), especially membrane-type 1 matrix metalloproteinase (MT1-MMP), which generates an active form of MMP-2 from proMMP-2, are deeply involved in angiogenesis as well as in tumor cell migration and metastasis. To obtain a specific inhibitor for MT1-MMP, we screened a number of natural and synthetic compounds using recombinant human MMP-2, MMP-7, and soluble MT1-MMP in a fluorogenic peptide cleavage assay. (−)-Epigallocatechin 3-O-gallate (EGCG) followed by (−)-epigallocatechin 3,5-di-O-gallate and epitheaflagallin 3-O-gallate, was found to have potent and distinct inhibitory activity against MT1-MMP. Therefore, we investigated the effect of EGCG on the suppression of MMP-2 activation as determined by gelatin zymography, and observed that the active form of MMP-2 in the conditioned medium of human umbilical vein endothelial cells was decreased in the presence of EGCG. The results suggest the possibility that tea polyphenols suppress tumor growth through the suppression of angiogenesis.
Databáze:	OpenAIRE
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