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Department of Life Science and Technology, Xinxiang Medical University, Xinxiang Henan 453003, P. R. China E-mail : yqh3499@yahoo.com.cn Fax : 86-373-3040015 Department of Experimental Center, Henan Institute of Science and Technology, Xinxiang Henan 453003, P. R. China School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, Henan Province, P. R. China Manuscript received online 02 April 2012, revised 11 April 2012, accepted 13 April 2012 High-performance liquid chromatography with diode-array detection (HPLC-DAD) has been evaluated for the separation and determination of quercetin in flowers of R. gallica and R. centifolia. The samples were extracted in 80% methanol by ultrasonic cleaning device for 40 min, and the content of quercetin was analyzed by HPLC under the optimal chromatographic conditions. A Eclipse XDB-C18 column (4.6 mm x 250 mm, 5 µm) was used as the analytical column. The mobile phase was methanol and (pH 4.2) phosphate buffer (v : v = 60 : 40) at a flow rate 0.8 mL/min at 25 °C. The detection wavelength was set at 372 nm to determine the content of quercetin. The results showed linearity over the range of 0.67-100 µg/mL, mean recoveries were in the range of 96.23-100.59% and RSDs was 0.27-0.96% for R. gallica and R. centifolia respectively. |
