Evaluation of real-time polymerase chain reaction assay for the diagnosis of mycobacterium tuberculosis complex
| Autor: | Constantin Marica, Georgeta Gilda Popescu, Adriana Socaci |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty Tuberculosis biology medicine.diagnostic_test business.industry medicine.disease biology.organism_classification Appropriate use Microbiology Mycobacterium tuberculosis Bronchoalveolar lavage Mycobacterium tuberculosis complex medicine Sputum REAL-TIME POLYMERASE CHAIN REACTION ASSAY medicine.symptom Culture negative business |
| Zdroj: | 10.2 Tuberculosis. |
| DOI: | 10.1183/13993003.congress-2015.pa2690 |
| Popis: | Background: The laboratory diagnosis of tuberculosis has long been based on microscopy and culture methods which had multiple limitations based on technical expertise and infrastructure limiting its use. Difficulties in identification and detection are addressed by nucleic acid amplification-detection methods which have been recently introduced in our country. Aims: We compared the performance of the COBAS TaqMan MTB assay against conventional Lowenstein-Jensen (L-J) culture and Mycobacterial growth indicator tube MGIT 960 an automated culture system for diagnosis of Mycobacterium tuberculosis. Methods: A total of 96 clinical specimens (72 respiratory and 24 nonrespiratory specimens) were collected from suspected TB between september and december 2014. Results: A total of 35 respiratory specimens (48.61%) were L-J culture positive and PCR. Of these, 32 (44.44%) were positive by MGIT. The other 37 specimens were culture negative, and two of them showed positive results by MGIT while one showed positive results by PCR. 10 nonrespiratory specimens (41.66%) were L-J culture positive and MGIT. Of these, 7 (29.16%) were positive by PCR. Ten cases yielded invalid results by PCR: 1 tissue samples, 3 sputum samples, 2 pleural fluids, 2 cerebrospinal fluids and 1 bronchoalveolar lavage. Conclusions: The performance of the COBAS TaqMan MTB test was comparable with the L-J culture and MGIT 960 in its performance as a diagnostic test in our study. This assay has an excellent performance in smear-positive respiratory specimens.However, further evaluation is required with a larger and varied sample for its appropriate use in clinical practice of our country. |
| Databáze: | OpenAIRE |
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