Vitrification negatively affects the Ca2+-releasing and activation potential of mouse oocytes, but vitrified oocytes are potentially useful for diagnostic purposes
| Autor: | Vanessa Thys, Petra De Sutter, Davina Bonte, Annekatrien Boel, Björn Heindryckx, Luc Leybaert |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030219 obstetrics & reproductive medicine Cryoprotectant Chemistry Endoplasmic reticulum Obstetrics and Gynecology Oocyte activation Oocyte Cryopreservation Andrology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology 0302 clinical medicine Human fertilization medicine.anatomical_structure Reproductive Medicine Ionomycin medicine Vitrification Developmental Biology |
| Zdroj: | Reproductive BioMedicine Online. 40:13-25 |
| ISSN: | 1472-6483 |
| DOI: | 10.1016/j.rbmo.2019.09.012 |
| Popis: | Research question To what extent does vitrification affect the Ca2+-releasing and activation potential of mouse oocytes, which are commonly used to determine the oocyte activation potential of human spermatozoa? Design The effect of mouse oocyte vitrification on Ca2+ dynamics and developmental competence after oocyte activation was assessed and compared with fresh mouse oocytes. Moreover, the Ca2+ store content of the endoplasmic reticulum was determined at different time points during the vitrification–warming procedure. Finally, the Ca2+ pattern induced by cryoprotectant exposure was determined. Results After human sperm injection into mouse oocytes, Ca2+ dynamics but not fertilization rates were significantly altered by vitrification warming (P 0.05), whereas activation rates after ionomycin exposure were significantly lower in vitrified–warmed oocytes (P 0.05). Cryoprotectant exposure was associated with a strong drop in endoplasmic reticulum Ca2+ store content. Oocytes rapidly recovered during warming and recovery in Ca2+-containing media; a threshold area under the curve of Ca2+ dynamics to obtain activation rates above 90% was determined. Conclusions Vitrified–warmed mouse oocytes display reduced Ca2+-releasing potential upon oocyte activation, caused by cryoprotectant exposure. With adapted classification criteria, these oocytes could be used for diagnosing oocyte activation deficiencies in patients. Evaluating the Ca2+-signalling machinery in vitrified–warmed human oocytes is required. |
| Databáze: | OpenAIRE |
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