Using internal transcribed spacers for Calanoida (Crustacea, Copepoda) species identification

Autor: Arturo B. Martinelli, Laura R. P. Utz, Luiz Fernando Loureiro Fernandes, Gustavo Martins Rocha
Rok vydání: 2018
Předmět:
Zdroj: Marine Biodiversity. 49:1565-1571
ISSN: 1867-1624
1867-1616
Popis: Mitochondrial gene Cytochrome Oxidase I (COI) is a standard marker for molecular identification of many animal groups, but its general use does not apply for calanoid copepods. This study aimed to test nuclear ribosomal internal transcribed spacer (ITS1 and ITS2) as barcode for this animal group. We sequenced ITS1, ITS2, and COI of 105 individuals representing 14 families of Calanoida collected in the western Atlantic for comparison of the successful sequencing rates. All GenBank available sequences of ITS1 (407 sequences) and ITS2 (665 sequences) were also used for analysis of their potential use as barcode and for the calculation of a genus per genus success identification rate. Sequencing rate efficiencies of ITS1 and ITS2 were far superior to those obtained for COI (98.1, 91.4, and 31.4%, respectively) showing high levels of universality for their primers. A barcode gap in ITS1 and ITS2 analyses was not observed, but overall success rates of identification were 99.07 and 98.45%, respectively. Efficiency of closely related species discrimination varied among different genera. We concluded that ITS1 and ITS2 presented better sequencing rates than COI for calanoid copepods and reinforced that it can be used as a helpful tool for species identification in this taxonomic group, after analyzing all available GenBank sequences until this date.
Databáze: OpenAIRE
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