Imaging of Insulitis in NOD Mice with IL-2-Gd-DTPA and 1.5 T MRI

Autor:	Pirjo Nuutila, Heikki Oivanen, Teemu Kalliokoski, Mikko Kankaanpää, Jani Saunavaara, Olli Simell, Anne Roivainen, Erkki Svedström
Rok vydání:	2011
Předmět:	Pathology medicine.medical_specialty business.industry Gadolinium chemistry.chemical_element General Medicine Nod medicine.disease medicine.anatomical_structure chemistry medicine Endocrine system business Pancreas Insulitis NOD mice
Zdroj:	Advances in Molecular Imaging. :43-49
ISSN:	2161-6752 2161-6728
Popis:	To explore the possibility to use 1.5 T MRI in imaging of accumulating gadolinium (Gd) into inflamed endocrine pancreas (insulitis), we compared intravenously delivered IL-2-Gd-DTPA and Gd-DTPA (Magnevist®) in phenotypically healthy 12 weeks old female non-obese diabetic (NOD) mice. At 1 to 48 h after the injection mice were sacrificed and the pancreas-to-background ratio was determined using MRI. In addition Gd concentration of the pancreas and plasma were measured. The pancreas-to-plasma ratio of Gd was 25-fold 24 h after the i.v. injection of IL-2-Gd-DTPA. At the same time the pancreas-to-background ratio measured with 1.5T MRI was 1.5-times higher in the NOD mice receiving IL-2-Gd-DTPA than Magnevist ® (271 ± 37 vs. 183 ± 0,1, P = 0.04) indicating that sufficient differences may exist between IL-2-Gd-DTPA accumulating in the pancreas and in the surrounding tissues to support the use of MRI for imaging of pancreatic insulitis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a85c3333e69369bbde02c8e3dcf3b429 https://doi.org/10.4236/ami.2011.13006 Zobrazit plný text záznamu