Effect of aerobic exercise on innate immune responses and inflammatory mediators in the spinal cord of diabetic rats
| Autor: | Abdol Hamid Habibi, Masoud Nikbakht, A. Kaki, Hadi Fathi Moghadam |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Physiology
Veterinary (miscellaneous) Endocrinology Diabetes and Metabolism Biophysics Inflammation Biochemistry 03 medical and health sciences 0302 clinical medicine Physiology (medical) Diabetes mellitus medicine Aerobic exercise Orthopedics and Sports Medicine 030304 developmental biology 0303 health sciences Innate immune system business.industry Spinal cord medicine.disease Pathophysiology medicine.anatomical_structure Neuropathic pain Immunology medicine.symptom business 030217 neurology & neurosurgery |
| Zdroj: | Comparative Exercise Physiology. 16:293-301 |
| ISSN: | 1755-2559 1755-2540 |
| Popis: | Neuronal inflammation is one of the pathophysiological causes of diabetes neuropathic pain. The purpose of this research was to determine the effect of aerobic exercise on innate immune responses and inflammatory mediators in the spinal dorsal horn in rats with diabetic neuropathic pain. 40 eight-week-old male Wistar rats (weight range 220±10.2 g) were randomly divided into four groups of (1) sedentary diabetic neuropathy (SDN), (2) training diabetic neuropathy (TDN), (3) training control (TC), and (4) sedentary control (SC). Diabetes was induced by injection of streptozocin (50 mg/kg). Following confirmation of behavioural tests for diabetes neuropathy, the training groups performed 6 weeks of moderate-intensity aerobic exercise on the treadmill. The expression of Toll like receptor (TLR)4, TLR2, tumour necrosis factor (TNF)-α, interleukin (IL)-1β and IL-10 genes in L4-L6 spinal cord sensory neurons was measured by Real Time PCR. Two-way ANOVA and Bonferroni’s post hoc tests were used for statistical analysis. After performing aerobic exercise protocol, the TDN compared to the SDN showed a significant decrease in the mean score of pain in the formalin test and a significant increase in the latency in Tail-Flick test was observed. The expression of TLR4, TLR2, TNF-α and IL-1β genes was significantly higher in the SDN than in the SC group (P |
| Databáze: | OpenAIRE |
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