| Popis: |
In this chapter we attempted to summarize recent reports on molecular biology and metabolism of bacteria belonging to the genus Gluconacetobacter with focus on its application for cellulose synthesis. The following issues were addressed: (1) the taxonomy of cellulose-producing Gluconacetobacter species; (2) cellulose synthase operons and flanking sequences from BNC producers, their role in biosynthesis of cellulose, and differences with other microorganisms; (3) recent advances in studies on three-dimensional structure of cellulose synthase subunits and their impact on Bacterial NanoCellulose (BNC) biosynthesis; (4) conditions of BNC production by Gluconacetobacter species from various substrates, including a variety of agro-industrial wastes; (5) metabolic pathways operating in Gluconacetobacter species verified as crucial for BNC synthesis by metabolic flux analysis; (6) the state of genome sequences data availability, concerning BNC-producing strains and their closest relatives; (7) genetic modifications of Ga. xylinus and Ga. hansenii and their effect on biosynthesis of cellulose with emphasis on methods and vectors used for these modifications. |
