Rapid Detection of Proliferating Cells Using Microwave Fixation and a Monoclonal Antibody to Bromodeoxyuridine
| Autor: | Marianne L. Kitchell, Julia J. Dibner |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Pathology
medicine.medical_specialty Histology Immunoperoxidase medicine.drug_class Biology Monoclonal antibody Rapid detection Molecular biology Staining Medical Laboratory Technology chemistry.chemical_compound Tissue sections chemistry Microwave irradiation medicine Anatomy Bromodeoxyuridine Fixation (histology) |
| Zdroj: | Journal of Histotechnology. 12:101-103 |
| ISSN: | 2046-0236 0147-8885 |
| DOI: | 10.1179/his.1989.12.2.101 |
| Popis: | The objective of this research was to develop a rapid morphological test for proliferating cells using diaminobenzidine as the substrate marker. This technique can be employed to visualize proliferating cells by using a monoclonal antibody to bromodeoxyuridine and a standard immunoperoxidase technique. This paper describes methods for the rapid demonstration of S-phase nuclei in routine paraffin tissue sections. The tissue was fixed by exposure to microwave irradiation for various times and temperatures. The subsequent staining results are reliable and can easily fit into an existing immunoperoxidase program. (The J Histotechnol 12:101, 1989.) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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