Biological generation of tritiated vitamin D3 metabolites and their purification by high-performance liquid chromatography

Autor:	L.J. Fraher, A. D. Dale, Thomas Clemens, J. L. H. O'riordan, C. J. Little
Rok vydání:	1980
Předmět:	Vitamin Chromatography Metabolite Organic Chemistry Clinical Biochemistry Substrate (chemistry) Biochemistry High-performance liquid chromatography In vitro Analytical Chemistry chemistry.chemical_compound chemistry Sephadex lipids (amino acids peptides and proteins)
Zdroj:	Chromatographia. 13:141-144
ISSN:	1612-1112 0009-5893
Popis:	A simple, reproducible method for the biological synthesis of tritiated 24,25-dihydroxycholecalciferol, 25,26-dihydroxycholecalciferol and 1,25-dihydroxycholecalciferol is described. Kidney homogenates from both vitamin D deficient and replete chicks were usedin vitro to generate these dihydroxylated metabolites using 25 (23,24-3H) hydroxycholecalciferol as the substrate. Tritiated products were purified by Sephadex LH 20 chromatography followed by high-performance liquid chromatography; the identity of each metabolite was established by chromatography with authentic crystalline preparations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a7a693f4b044b51ba490246c42a06d6f https://doi.org/10.1007/bf02259302 Zobrazit plný text záznamu Full text from SpringerLink