A new homozygous typing cell with HLA-D'H' (DB6) specificity

Autor: Arne Svejgaard, Lars P. Ryder, Bodil K. Jakobsen, P. Platz
Rok vydání: 2008
Předmět:
Zdroj: Tissue Antigens. 27:285-290
ISSN: 1399-0039
0001-2815
DOI: 10.1111/j.1399-0039.1986.tb01534.x
Popis: A new homozygous typing cell (HTC), SK is described. Mixed leukocyte culture (MLC) showed that SK is homozygous for HLA-D"H" (DB6) defined by the HTC, Herluf, and that the HLA-D typing results obtained by Herluf and SK are highly significantly correlated (Kendall's R = 0.46, p = 2.5 X 10(-5)). Both Herluf and SK are also homozygous for a new class II determinant, DN-1, defined by the monoclonal anti-B-lymphocyte antibody, 9w925, developed by Aizawa. The corresponding DN-1 antigen was present in 2.2% of 136 random, unrelated Danes and in all of six unrelated HLA-D"H" positive but in none of 20 HLA-D"H" negative individuals. Thus, there is an absolute and highly significant (p = 4 X 10(-6)) association between the cellularly defined HLA-D"H" determinant and the serologically defined DN-1 antigen, which strongly suggests that HLA-D"H" can now be detected serologically. DN-1 may be identical to DRw12 which is, however, poorly defined. The HTC-donor SK was immunized by pregnancy, and her serum contains anti-HLA-B7, which can easily be absorbed, and anti-B-lymphocyte antibodies which reacted with cells from 87.7% of 536 unrelated Danes. The reaction pattern of this serum is negatively associated with DR3, w6, and w8. This serum may define a new broad, cross-reacting antigen belonging to the same group as DRw52 and DRw53 or DQw1-w3, but it is clearly different from each of these antigens.
Databáze: OpenAIRE
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