Using AFM to image the internal structure of starch granules
| Autor: | A.P. Gunning, Michael J. Ridout, R. H. Wilson, Mary L. Parker, V.J. Morris |
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| Rok vydání: | 2002 |
| Předmět: |
Araldite
Materials science Polymers and Plastics Starch Organic Chemistry Granule (cell biology) technology industry and agriculture Analytical chemistry food and beverages engineering.material law.invention chemistry.chemical_compound chemistry Coating Optical microscope Chemical engineering law Materials Chemistry engineering Ultrastructure Electron microscope Potato starch |
| Zdroj: | Carbohydrate Polymers. 50:123-132 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/s0144-8617(02)00021-8 |
| Popis: | Atomic force microscopy (AFM) has been used to observe the ultra-structure of starch granules for starches from different botanical sources (maize and potato). Starch granules were embedded, sectioned and imaged in dc constant force topographic, error signal and force modulation modes. AFM images of the starches embedded in Nanoplast®, a resin previously used for AFM and electron microscopy studies, failed to reveal the growth ring structure within the granules consistently. Furthermore, ultra-structural features could not unequivocally be attributed to the ‘blocklet’ structure of the granule because of the underlying granular texture of the resin itself. In contrast the use of rapid setting Araldite, a non-penetrating resin, allowed the ultra-structure of the granules to be viewed without the necessity of pre-treatment (lintnerisation or enzymatic degradation) steps. The images demonstrated clearly the importance of the choice of embedding material, and showed that under the correct conditions ‘growth rings’ and blocklet structures can be observed in ‘near native’ granules. |
| Databáze: | OpenAIRE |
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