Real-Time Endocytosis Measurements by Membrane Capacitance Recording at Central Nerve Terminals
| Autor: | Xuelin Lou |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Membrane potential Materials science Endocytosis Capacitance Exocytosis Cell membrane 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Frequency domain medicine Biophysics Patch clamp Calyx of Held 030217 neurology & neurosurgery |
| Zdroj: | Clathrin-Mediated Endocytosis ISBN: 9781493987177 |
| DOI: | 10.1007/978-1-4939-8719-1_8 |
| Popis: | Endocytosis is fundamental to cell function. It can be monitored by capacitance measurements under patch-clamp recordings. Membrane capacitance recording measures the cell membrane surface area and its changes at high temporal-resolution and sensitivity, and it is a powerful biophysical approach in the field of exocytosis and endocytosis. A popular one is the frequency domain method that entails processing passive sinusoidal membrane currents induced by a sinusoidal voltage. This technique requires a phase-sensitive detector or "lock-in amplifier" implemented in hardware or software during patch-clamp recordings. It has been widely used in many secretory cells, but its application directly at central presynaptic terminals is technically challenging. We have applied this technique to study synaptic endocytosis in the calyx of Held, a large glutamatergic synaptic terminal, as well as mouse pancreatic β-cells. The presynaptic capacitance measurements provide a unique alternative to measuring transmitter release and presynaptic endocytosis. Here, we describe this method at the calyx of Held in acute brain slices and provide a practical guide to obtaining high quality capacitance measurements at presynaptic terminals. |
| Databáze: | OpenAIRE |
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