Effect of supplemented sericin on the development, cell number, cryosurvival and number of lipid droplets in cultured bovine embryos
| Autor: | Yuichi Hasegawa, Sueo Niimura, Kei Imai, Yasushi Inaba, Kenji Kajitani, Yutaka Hashiyada, Hidetoshi Teramoto, Mamoru Irie, Toru Takahashi, Misa Hosoe |
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| Rok vydání: | 2016 |
| Předmět: |
030219 obstetrics & reproductive medicine
Chemistry 0402 animal and dairy science Embryo 04 agricultural and veterinary sciences General Medicine 040201 dairy & animal science Sericin In vitro Andrology 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure In vivo Lipid droplet embryonic structures medicine sense organs Bovine embryo Blastocyst General Agricultural and Biological Sciences Fetal bovine serum |
| Zdroj: | Animal Science Journal. 88:241-247 |
| ISSN: | 1344-3941 |
| DOI: | 10.1111/asj.12628 |
| Popis: | Sericin was investigated as an alternative to fetal bovine serum (FBS) for bovine embryo culture. In vitro matured oocytes were developed using 0.05%, 0.1% or 0.15% sericin. The developmental rate, cryosurvival rate and blastulation time of these embryos were compared with those of embryos developed using 5% FBS. The number of lipid droplets was compared among the blastocysts developed using 5% FBS, using 0.05% sericin and in vivo. The rate of cleavage and blastocyst formation was similar among all groups. Blastulation occurred significantly earlier in the embryos developed using 5% FBS than in those developed using sericin at any concentration (P |
| Databáze: | OpenAIRE |
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