An ultrasonic inactivation of Aspergillus niger glucose oxidase in aqueous solutions

Autor:	E. I. Karaseva, E. I. Tarun, D. I. Metelitsa
Rok vydání:	2009
Předmět:	Octanol Aqueous solution biology Chemistry Butanol Radical Sonication Aspergillus niger biology.organism_classification Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound medicine biology.protein Organic chemistry Glucose oxidase Mannitol Nuclear chemistry medicine.drug
Zdroj:	Applied Biochemistry and Microbiology. 45:9-16
ISSN:	1608-3024 0003-6838
Popis:	The inactivation of Aspergillus niger glucose oxidase (GO) was studied in 0.02 M phosphate-citrate buffer (PCB) at various pH, temperatures of 37–59°C, and sonication with low frequency (27 kHz, LF-US) and high frequency (2.64 MHz, HF-US) ultrasound. The GO inactivation was characterized by the effective first-order inactivation rate constantsk in, k*in andk in(us), reflecting the total, thermal, and ultrasonic inactivation components. The constants strongly depended on the pH and temperature of solution, GO concentration, and the presence of acceptors of the free radicals HO·—DMF, DMSO, ethanol, butanol, octanol, and mannitol, confirming that the active radicals formed in the ultrasonic cavitation field played an important role in the GO inactivation. The activation energy in the loss of GO catalytic activity considerably decreased when the enzyme solution was treated with LF-US or HF-US. The dissociative scheme of GO inactivation is discussed. Mannitol can be used for protection of GO from inactivation with LF-US or HF-US in the food industry and immunobiotechnology.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a46b0ee050a7327cefb973027145b74a https://doi.org/10.1134/s0003683809010025 Zobrazit plný text záznamu