| Autor: |
Goto Hideaki, Syuuji Shimazaki, Ogo Ken, Wada Takako, Koizumi Takeo, Miyauchi Hiroshi, Tanaka Hideharu, Taketo Matsuda, Yamaguchi Ryo, Enoki Mika, Takami Yoshihiro |
| Rok vydání: |
2004 |
| Předmět: |
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| Zdroj: |
Wound Repair and Regeneration. 12:A11-A11 |
| ISSN: |
1067-1927 |
| DOI: |
10.1111/j.1067-1927.2004.abstractan.x |
| Popis: |
Aim: Available donor skin is so limited in extensive burns that wounds are usually closed using STSG harvested repeatedly from unburned skin. In such cases, it is important to accelerate the epithelization process of the donor wounds. We have developed a cultured cellular sheet composed of mixture of keratinocytes (KC) and fibroblasts (FB) seeded on a polyurethane membrane (mixed culture sheet) and investigated its effectiveness for the wound healing of STSG donor wounds. Methods: Normal human KC and FB were primarily cultured from a surplus human skin in a skin graft surgery. After a few passages of the cultivation, both cells were seeded on a thin polyurethane membrane. Each seeding density of KC and FB were equal. Several days after the co-cultivation, the mixed culture sheet was transferred to a freshly harvested STSG donor wound in an extensive burn patient. A polyurethane membrane without cells was used for control coverage. Seven patients were treated using the mixed culture sheets. Autologous cells were used in 3 cases, and allogenic cells were used in the other 4 cases. The time for the final epithelization was determined. In vitro production of several growth factors by a cellular sheet was also investigated by ELASA. Results and Conclusion: TGF-alpha, EGF, TGF-beta, VEGF were well produced by the mixed culture sheet. The time for final epithelization of the wounds was reduced by 2.5 days with the mixed culture sheet application as compared to the control sites. It was suggested that the mixed culture sheet may be useful for the treatment of STSG donor wounds. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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