| Autor: |
Hojin Ryu, Hyeongmin Kim, Hyemin Kim, Tae Kyung Hyun, Jinsu Lee, Seung-Jae Lee, Ick-Hyun Jo, Kyungho Won, Wonsil Bae |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
The Plant Pathology Journal. 33:362-369 |
| ISSN: |
1598-2254 |
| DOI: |
10.5423/ppj.oa.03.2017.0046 |
| Popis: |
White root rot disease, caused by the pathogen Rosellinia necatrix, is one of the world's most devastating plant fungal diseases and affects several commercially important species of fruit trees and crops. Recent global outbreaks of R. necatrix and advances in molecular techniques have both increased interest in this pathogen. However, the lack of information regarding the genomic structure and transcriptome of R. necatrix has been a barrier to the progress of functional genomic research and the control of this harmful pathogen. Here, we identified 10,616 novel full-length transcripts from the filamentous hyphal tissue of R. necatrix (KACC 40445 strain) using PacBio single-molecule sequencing technology. After annotation of the unigene sets, we selected 14 cell cycle-related genes, which are likely either positively or negatively involved in hyphal growth by cell cycle control. The expression of the selected genes was further compared between two strains that displayed different growth rates on nutritional media. Furthermore, we predicted pathogen-related effector genes and cell wall-degrading enzymes from the annotated gene sets. These results provide the most comprehensive transcriptomal resources for R. necatrix, and could facilitate functional genomics and further analyses of this important phytopathogen. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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