Isolation and characterization of stress induced Ty1-copia like retrotransposable elements in chickpea (Cicer arietinum L.)

Autor:	K. C. Upadhyaya, M. K. Rajput
Rok vydání:	2010
Předmět:	Genetics Abiotic stress fungi Biophysics food and beverages Retrotransposon Molecular cloning Biology Molecular biology Reverse transcriptase Structural Biology Transcription (biology) GenBank Gene expression Gene
Zdroj:	Molecular Biology. 44:693-698
ISSN:	1608-3245 0026-8933
Popis:	Almost all active plant retroelements are known to be induced by various biotic and abiotic stresses. Here, we show the presence of transcriptionally active Ty1-copia like retroelements in chickpea and their induction in response to abiotic stress. Eight Ty1-copia retrotransposon like mRNA sequences were reverse transcribed, amplified, cloned and characterized from stressed plants. These mRNA sequences were not detected in chickpea plants grown under normal conditions. Basing on the similarity analysis, these RT transcript sequences were classified into three families. It is proposed that all sequences except CARE7 might be transcript sequences of functional retrotransposons. The mRNA sequence CARE3 shows 99% nucleotide identity to a genomic Ty1-copia like sequence present in the Genbank with accession no. AJ535883.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a29c9b619709f383263dfae453b394bf https://doi.org/10.1134/s0026893310050031 Zobrazit plný text záznamu Full text from SpringerLink