Effect of 1,25(OH)2D3and 20(OH)D3on interleukin-1β-stimulated interleukin-6 and -8 production by human gingival fibroblasts

Autor: Sidney H. Stein, V. Nakashyan, David A. Tipton, Anastasios Karydis, Rania Livada
Rok vydání: 2017
Předmět:
Zdroj: Journal of Periodontal Research. 52:832-841
ISSN: 0022-3484
DOI: 10.1111/jre.12452
Popis: Background and Objective Vitamin D—1,25(OH)2D3 or 1,25D3—maintains healthy osseous tissue, stimulates the production of the antimicrobial peptide cathelicidin and has anti-inflammatory effects, but it can cause hypercalcemia. Evidence links diminished serum levels of 1,25D3 with increased gingival inflammation. Periodontitis progression is associated with increased local production of inflammatory mediators by immune cells and gingival fibroblasts. These include interleukin (IL)-6, a regulator of osteoclastic bone resorption, and the neutrophil chemoattractant IL-8, both regulated by signaling pathways, including NF-κB and MAPK/AP-1. The objectives were to determine the effects of 1,25D3 or a non-calcemic analog, 20-hydroxyvitamin D3—20(OH)D3 or 20D3—on IL-1β-stimulated IL-6 and IL-8 production, and NF-κB and MAPK/AP-1 activation, by human gingival fibroblasts. Material and Methods Human gingival fibroblasts were incubated ± IL-1β, with or without exposure to 1,25D3 or 20D3. IL-6 and IL-8 in culture supernatants were measured by enzyme-linked immunosorbent assay. NF-κB (p65) and AP-1 (phospho-cJun) and were measured in nuclear extracts via binding to specific oligonucleotides. Data were analyzed using ANOVA and Scheffe's F procedure for post hoc comparisons. Results IL-1β-stimulated IL-6 and IL-8 levels were both significantly inhibited (40%-60%) (P
Databáze: OpenAIRE
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