The Simian Immunodeficiency Virus (SIV) rev Gene Regulates Env Expression
Autor: | Vanessa M. Hirsch, Sheau-Mei Cheng, Shaw-Guang Lee, Paul P. Hung, Marlene Blume, Philip R. Johnson |
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Rok vydání: | 1990 |
Předmět: | |
Zdroj: | Journal of Medical Primatology. 19:167-176 |
ISSN: | 1600-0684 0047-2565 |
Popis: | The rev gene product is a trans-acting nuclear regulatory protein that is essential for AIDS virus replication. To define the effect of the SIV rev gene on its gp160 expression, two SV40 constructs have been made: pBC17 is a rev+env+ construct, and pBD21 is a rev-env+ construct. After transfecting the constructs into COS-1 cells, RNA, protein, syncytium formation, and monoclonal antibody blocking assays were performed. The results indicated that the SIV rev gene positively influenced the level of full-length env mRNA and was required for expression of SIV gp160. |
Databáze: | OpenAIRE |
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