The Simian Immunodeficiency Virus (SIV) rev Gene Regulates Env Expression

Autor:	Vanessa M. Hirsch, Sheau-Mei Cheng, Shaw-Guang Lee, Paul P. Hung, Marlene Blume, Philip R. Johnson
Rok vydání:	1990
Předmět:	Regulation of gene expression General Veterinary viruses virus diseases RNA Simian immunodeficiency virus Biology medicine.disease_cause Virology Virus Gene product Viral replication Gene expression medicine Animal Science and Zoology Gene
Zdroj:	Journal of Medical Primatology. 19:167-176
ISSN:	1600-0684 0047-2565
Popis:	The rev gene product is a trans-acting nuclear regulatory protein that is essential for AIDS virus replication. To define the effect of the SIV rev gene on its gp160 expression, two SV40 constructs have been made: pBC17 is a rev+env+ construct, and pBD21 is a rev-env+ construct. After transfecting the constructs into COS-1 cells, RNA, protein, syncytium formation, and monoclonal antibody blocking assays were performed. The results indicated that the SIV rev gene positively influenced the level of full-length env mRNA and was required for expression of SIV gp160.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::a1b44f0284b0b49ecb57f6990095aee9 https://doi.org/10.1111/j.1600-0684.1990.tb00425.x Zobrazit plný text záznamu