COMPARISON OF THE APPLICATION OF DOMESTIC «ELISA-IGM-DENGUE» KIT, DELIVERED IN THE D.I. IVANOVSKY INSTITUTE OF VIROLOGY (MOSCOW, RUSSIAN FEDERATION) AND «ANTI-DENGUE VIRUS ELISA IGM» KIT (EUROIMMUN, GERMANY) FOR THE SERODIAGNOSIS OF DENGUE FEVER
| Autor: | S. G Mardanly, M. A. Saifullin, V. F. Larichev, A. M. Butenko, Yu. A Akinshina |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
biology business.industry Congenital cytomegalovirus infection Rubella virus Dengue virus medicine.disease_cause medicine.disease Virology Virus Dengue fever 03 medical and health sciences Titer 030104 developmental biology Herpes simplex virus medicine biology.protein Antibody business |
| Zdroj: | Epidemiology and Infectious Diseases. 22:4-8 |
| ISSN: | 2411-3026 1560-9529 |
| DOI: | 10.17816/eid40949 |
| Popis: | 88 sera from patients with dengue fever were examined with the use of two test systems: Pilot Production kit «ELISA- IgM-dengue», delivered in the D.I. Ivanovsky Institute of Virology (Russian Federation) and. All 88 samples were positive for specific IgM in «ELISA-IgM-dengue», but 8 cases examined with the test kit «Anti-Dengue virus ELISA (IgM)» (Euroimmun, Germany) appeared to be negative. The ratio between the titers of anti-dengue IgM determined in patients’ blood samples by "ELISA-IgM-dengue" and «Ratio» (index, recommended by the company «Euroimmun» for differentiation of positive, equivocal or negative results, was evaluated. 53 blood sample containing M antibodies to cytomegalovirus (n = 43), Epstein-Barr virus (n = 6), herpes simplex virus (n = 2) and rubella virus (n = 2) were tested by both ELISA kits to compare their specificity. When «ELISA- IgM-dengue» kits were applied all observed samples were negative, but under the application of the «Anti-Dengue virus ELISA (IgM)» kit (Euroimmun, Germany) 17 samples (32.1%) were false positive or equivocal. |
| Databáze: | OpenAIRE |
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