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Metastatic breast cancer is one of the leading causes of cancer mortality worldwide. While many therapeutic strategies try to control and combat this disease, a better understanding of cellular communication within the acidic tumour microenvironment is needed to improve treatment success. Here we characterized the secretory profile of MCF-7 breast cancer cells in medium of physiological pH, and compared it to those cells treated with medium of 6.5 and 3.2. MCF-7 cells secreted IP-10, VEGF, EGF, FGF, G-CSF, GDF-15, and myoglobin at baseline. When treated with acidic medium, MCF-7 cells downregulated secretion of many of these factors, although myoglobin and GDF-15 were significantly increased at pH 6.5 (1hr) (p |
