Production and Characterization of MIC1: A Lectin from Toxoplasma gondii
| Autor: | Rafael Ricci-Azevedo, Sandra Maria de Oliveira Thomaz, Maria Cristina Roque-Barreira, Flávia Costa Mendonça-Natividade |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030103 biophysics biology Chemistry Heterologous Lectin Toxoplasma gondii medicine.disease_cause biology.organism_classification Inclusion bodies law.invention Sialic acid Microneme 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Biochemistry law parasitic diseases medicine biology.protein Recombinant DNA Escherichia coli |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071604298 |
| Popis: | Some lectins of pathogens interact with host cells through the recognition of specific carbohydrates displayed on the mammals' cell surface. The microneme protein 1 (MIC1) from Toxoplasma gondii has a lectin domain that specifically binds sialic acid residues, often found in the terminal positions of N-glycans of mammalian cells. The necessary studies on the MIC1 biological roles have been limited initially by the laborious purification of the protein from T. gondii tachyzoites and the low yields verified. Then Escherichia coli has been transformed with a construct containing the MIC1 gene, and the obtained recombinant MIC1 (rMIC1) has been purified from the inclusion bodies. Herein, we detail the methodology of heterologous production and purification of rMIC1 and protocols to assay the rMIC1 lectin ability. |
| Databáze: | OpenAIRE |
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