Osmotic Characteristics of Mouse Spermatozoa in the Presence of Extenders and Sugars1
| Autor: | Michael A. Byers, Erik J. Woods, John K. Critser, Julie Gilmore, Yuksel Agca, Jun Liu |
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| Rok vydání: | 2002 |
| Předmět: | |
| Zdroj: | Biology of Reproduction. 67:1493-1501 |
| ISSN: | 1529-7268 0006-3363 |
| Popis: | Successful cryopreservation requires cells to tolerate volume excursions experienced during permeating cryoprotectant equilibration and during cooling and warming. However, prior studies have demonstrated that mouse spermatozoa are extremely sensitive to osmotically induced volume changes. A series of three experiments were conducted 1) to test the efficacy of two commonly used extender media components, egg yolk (EY) and skim milk (SM), in broadening the osmotic tolerance limits (OTL) of ICR and B6C3F1 murine spermatozoa; 2) to determine if the extender components affected sperm plasma membrane permeability coefficients for water and cryoprotective agent (CPA) characteristics; and 3) to test the effects of permeating and nonpermeating CPA on mouse sperm morphology. In experiment 1, sperm samples were added to 150, 225, 300, 450, or 600 mOsm NaCl, EY, SM, sucrose, or choline chloride at 22°C and then returned to isosmotic conditions. In experiment 2, epididymal sperm were preequilibrated in 1 M g... |
| Databáze: | OpenAIRE |
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