The determination of uric acid in biological fluids: I. A modification of the method of Bergmann and Dikstein

Autor: Martha L. Wharton, D. R. A. Wharton
Rok vydání: 1960
Předmět:
Zdroj: Analytical Biochemistry. 1:213-217
ISSN: 0003-2697
DOI: 10.1016/0003-2697(60)90047-6
Popis: A simplified, generally applicable modification of Bergmann and Dikstein's method for uric acid analysis is proposed which has been verified by means of Kalckar's uricase method when applied to urine and blood plasma. Recovery of 95.1 ± 3.4% has been obtained with 5 μg uric acid/ml added to an extract of cockroach feces containing 3 μg uric acid/ml, and equally good results with the addition of 2.5 or 1.25 μg. Similar recoveries were obtained from urine and plasma.
Databáze: OpenAIRE