Autor: |
V. Lunyak, L. Noskin, N. Giliano, R. Burgess |
Rok vydání: |
2003 |
Předmět: |
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Zdroj: |
Molecular Biology. 37:140-147 |
ISSN: |
0026-8933 |
Popis: |
A modified version of the chromosomal immunoprecipitation (ChIP) assay was implemented for discrete isolation and characterization of actively transcribed genes. Specifically, it was demonstrated with the gene II/9-1 of Sciara coprophila as a model locus that significant enhancement in the isolation of actively transcribed versus repressed and inactive genes can be achieved through the ChIP methodology. A combination of solid-phase magnetic bead technology with chromosomal immunoprecipitation using antibodies that recognize the large subunit (c) of RNA polymerase II resulted in efficient isolation of the promoter region of gene II/9-1 exclusively during the amplification stage of larval development, when the gene is actively transcribed. It is postulated that the novel technology described herein can be applied to a wide variety of systems for efficient isolation and in vivo assessment of actively transcribed genes regulated by virtually any given transcription factor. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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