Field-based Evaluation of a Novel SPME-GC-MS Method for Investigation of Below-ground Interaction between Brassica Roots and Larvae of Cabbage Root Fly, Delia radicum L
| Autor: | Colin J. Alexander, K. Andrew Evans, A. Nicholas E. Birch, Tom Shepherd, William Deasy |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Larva Plant roots biology Chemistry fungi Brassica Spme gc ms Plant Science General Medicine biology.organism_classification 010603 evolutionary biology 01 natural sciences Biochemistry Analytical Chemistry Horticulture Complementary and alternative medicine Drug Discovery Molecular Medicine Field based Gas chromatography–mass spectrometry Delia radicum 010606 plant biology & botany Food Science Field conditions |
| Zdroj: | Phytochemical Analysis. 27:343-353 |
| ISSN: | 0958-0344 |
| DOI: | 10.1002/pca.2634 |
| Popis: | Introduction Collection of volatiles from plant roots poses technical challenges due to difficulties accessing the soil environment without damaging the roots. Objectives To validate a new non-invasive method for passive sampling of root volatiles in situ, from plants grown under field conditions, using solid phase micro-extraction (SPME). Methods SPME fibres were inserted into perforated polytetrafluoroethene (PTFE) tubes positioned in the soil next to broccoli plants for collection of root volatiles pre- and post-infestation with Delia radicum larvae. After sample analysis by gas chromatography-mass spectrometry (GC-MS), principal component analysis (PCA) was applied to determine differences in the profiles of volatiles between samples. Results GC-MS analysis revealed that this method can detect temporal changes in root volatiles emitted before and after Delia radicum damage. PCA showed that samples collected pre- and post-infestation were compositionally different due to the presence of root volatiles induced by D. radicum feeding. Sulphur containing compounds, in particular, accounted for the differences observed. Root volatiles emission patterns post-infestation are thought to follow the feeding and developmental progress of larvae. Conclusion This study shows that volatiles released by broccoli roots can be collected in situ using SPME fibres within perforated PTFE tubes under field conditions. Plants damaged by Delia radicum larvae could be distinguished from plants sampled pre-infestation and soil controls on the basis of larval feeding-induced sulphur-containing volatiles. These results show that this new method is a powerful tool for non-invasive sampling of root volatiles below-ground. Copyright © 2016 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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