| Popis: |
This study investigated the claims of traditional practitioners in the use of Anthocleista djalonensis for the treatment of various diseases and infections in Benue State, Nigeria. The leaves of the plant were collected; air dried; pulverized and successively extracted using hexane, ethyl acetate, ethanol and methanol by microwave assisted method. The phytochemical analysis of the leaf extracts of Anthocleista djalonensis revealed the presence of glycosides, saponins, terpenes, sterols flavonoids, anthraquinones, resins and balsams in Anthocleista djalonensis leaf. The antimicrobial screening of the hexane, ethyl acetate, ethanol and methanol extracts were carried out on Methicillin Resistant Staphylococcus aureus, Vancomycin Resistant enterococci, Staphylococcus aureus, Helicobacter pylori, Candida albicans, Candida krusei, Candida tropicalis, Escherichia coli, Proteus mirabilis, and Pseudomonas aeruginosa using agar-well diffusion method. The antimicrobial studies showed that all the extracts exhibited activities against Methicillin Resistant Staphylococcus aureus, Vancomycin Resistant enterococci, S. aureus, H. pylori, C. albicans, and C. krusei with significant zones of inhibition ranging from 16 - 20 mm for hexane extract, 22 - 28 mm for ethyl acetate extract, 20 - 24 mm for ethanol extract and 20 - 23 mm for methanol extract against test microbes. E. coli, P. aeruginosa, P. mirabilis and C. tropicalis showed resistance to the extracts; Minimum Inhibitory Concentration (MIC) of the extracts against the stated microbes were 5 mg/mL, 5 mg/mL, 5 mg/mL, 5 mg/mL, 2.5 mg/mL and 5 mg/mL respectively for hexane extract. 1.25 mg/mL, 1.25 mg/mL, 0.62 mg/mL, 0.62 mg/mL 0.62 mg/mL and 1.25 mg/mL respectively for ethyl acetate extract; Ethanol and methanol extracts recorded 1.25 mg/mL against all the stated test microbes. The minimum bactericidal/fungicidal concentration of the extracts against Methicillin Resistant Staphylococcus aureus, Vancomycin Resistant enterococci, S. aureus, H. pylori, C. albicans and C. krusei ranged from 5 mg/mL to 1.25 mg/mL. The results support the use of Anthocleista djalonensis in traditional medicine. |
