| Autor: |
Daniel Neu, Gary C. Visor, Jeffrey S. Fleitman |
| Rok vydání: |
1986 |
| Předmět: |
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| Zdroj: |
Journal of AOAC INTERNATIONAL. 69:20-24 |
| ISSN: |
0004-5756 |
| DOI: |
10.1093/jaoac/69.1.20 |
| Popis: |
A reverse phase liquid chromatographic (LC) procedure is described for quantitating oxfendazole (2-(methoxycarbonylamino)-5-phenyIsuIfinylbenzimidazole)) in swine premix. Sample preparation consists of extracting oxfendazole with an acetone-methanol mixture. An aliquot of the extract is then centrifuged to separate undissolved premix excipients. Internal standard is added to the supernate and the sample is further diluted with water-acetonitrile-phosphoric acid (80 + 20 + 1). Oxfendazole is quantitatively determined using a Partisil-5-ODS-3 column with acetonitrile-O.OlM phosphate buffer (pH 6.0) as the mobile phase. The method is stability specific and yields a mean recovery of 101.1 ± 0.4% for the 1.35% premix formulation. The dependence of chromatographic performance characteristics on mobile phase organic content, pH, and buffer concentration is also reported. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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