DNA recovery from environmental samples on suspension columns under a combined action of ultrasound and magnetic fields followed by polymerase chain reaction detection
| Autor: | Boris Ya. Spivakov, Valeriy M. Shkinev, Dmitriy G. Petrov, Rustam Kh. Dzhenloda |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Chromatography
Chemistry business.industry 010401 analytical chemistry Ultrasound Analytical chemistry 02 engineering and technology General Chemistry Dna recovery 021001 nanoscience & nanotechnology 01 natural sciences DNA extraction 0104 chemical sciences Magnetic field law.invention chemistry.chemical_compound law 0210 nano-technology Suspension (vehicle) business Polymerase chain reaction DNA |
| Zdroj: | Mendeleev Communications. 27:302-303 |
| ISSN: | 0959-9436 |
| DOI: | 10.1016/j.mencom.2017.05.029 |
| Popis: | A new method is proposed for dynamic DNA extraction from environmental samples (soil, water) on suspension columns containing silica-coated magnetic sorbents (Fe 3 O 4 @SiO 2 ) under a combined influence of ultrasound and magnetic fields. The recovered DNA was subsequently determined by real-time polymerase chain reaction on an ANK-32 device. It has been shown for the first time that the suspension columns enable the preconcentration of DNA (taking Mycobacterium tuberculosis as an example) in a dynamic mode from large volumes of water and soil extracts (100 ml and more) to be performed. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect