The effect of dipyridamole embedded in a drug delivery system made by electrospun nanofibers on aortic endothelial cells
| Autor: | Alexandros Repanas, Anne Klett, Birgit Glasmacher, Anaclet Ngezahayo, Almke Bader |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Materials science Cell growth technology industry and agriculture Gap junction Pharmaceutical Science Nanotechnology Biological activity 02 engineering and technology 021001 nanoscience & nanotechnology Electrospinning Dipyridamole 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Polycaprolactone Drug delivery Biophysics medicine Platelet aggregation inhibitor 0210 nano-technology medicine.drug |
| Zdroj: | Journal of Drug Delivery Science and Technology. 35:343-352 |
| ISSN: | 1773-2247 |
| Popis: | An important sector of biomedical research is the generation of drug delivery systems (DDSs), which can be used alone or integrated in stents for sustained drug release. In this context, by encapsulating the platelet aggregation inhibitor dipyridamole (DIP) in polycaprolactone (PCL) we tested whether we could generate a DDS with PCL by electrospinning. By characterizing the fibers' structural properties we found that fibers' diameters were affected by the concentration of DIP, which consequently had an impact on the surface area-to-volume ratio and therefore to cumulative drug release. In vitro cell studies on a bovine aortic endothelial cell line showed that PCL itself did not affect the cells according to analysis of the number of viable cells over time and metabolic activity. We found that DIP which was released from the electrospun fibers in the solution was accumulated in the cells and that it had similar effects on the cells as it had directly delivered to them. DIP inhibited the cell growth and enhanced the gap junction coupling of the endothelial cells. The results indicate that the encapsulation and the electrospinning did not affect the biological activity of DIP and show that the method can be used to generate a DDS. |
| Databáze: | OpenAIRE |
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