Callus formation and plant regeneration from tubercles of Coryphantha elephantidens (Lem.) Lem

Autor:	Brijmohan Singh Bhau, A. K. Wakhlu
Rok vydání:	2000
Předmět:	Callus formation Plant Science Biology chemistry.chemical_compound Light intensity Murashige and Skoog medium chemistry Callus Shoot Botany Kinetin Pith Subculture (biology) Biotechnology
Zdroj:	In Vitro Cellular & Developmental Biology - Plant. 36:211-214
ISSN:	1475-2689 1054-5476
DOI:	10.1007/s11627-000-0039-x
Popis:	Callus cultures were established from pith tissue of Coryphantha elephantidens (Lem.) Lem. on Murashige and Skoog (MS) basal medium supplemented with 9.05 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 2.3 μM kinetin. Highest shoot regeneration frequency was observed on a medium containing 6.9 μM kinetin and 2.3 μM 2,4-D under 30 μE m−2 s−1 light intensity with a 16-h photoperiod. Calluses retained organogenic potential throughout several passages of subculture (18 mo.). Shoots were rooted on MS medium without plant growth regulators. All (100%) plantlets transplanted to soil survived acclimatization. Regenerated plants showed good overall growth and were morphologically similar to the mother plants.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9d1ec51ff83b5039cd02307fe79c89bb https://doi.org/10.1007/s11627-000-0039-x Zobrazit plný text záznamu Full text from SpringerLink