Diverse VH and VL genes are used to produce antibodies against a defined protein epitope

Autor:	M L Andria, S Levy, E Benjamini
Rok vydání:	1990
Předmět:	Immunology Immunology and Allergy
Zdroj:	The Journal of Immunology. 144:2614-2619
ISSN:	1550-6606 0022-1767
Popis:	mAb secreting hybridomas were produced from mice hyperimmune to the model Ag tobacco mosaic virus protein. Six mAb were selected for their ability to bind synthetic peptides corresponding to amino acid residues 103-112 and 97-107 of tobacco mosaic virus protein. These mAb were analyzed for their fine specificity by measuring binding to synthetic analogs of the decapeptide, and cDNA sequences encoding the mAb V regions were determined. These analyses revealed that a wide range of different V regions are capable of binding with the same decapeptide epitope, and that these antibody sequence differences generally coincided with different binding fine specificities. This diverse antibody response with specificity for the same epitope demonstrates both the breadth of potential of the immune system and the lack of exclusivity in specific protein:protein interactions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9cd84994c23a544ee7df4b3806c55214 https://doi.org/10.4049/jimmunol.144.7.2614 Zobrazit plný text záznamu