The detection of single-stranded RNA in an isometric virus-like particle from Shiitake mushroom [Lentinus edodes (Berk.) Sing.]

Autor:	Chen Mingjie, Pan Yangjie, Shen Xueren, Shen Juying, Gong Zuxun
Rok vydání:	1993
Předmět:	Gel electrophoresis Mushroom biology RNase P viruses fungi RNA RNA virus biology.organism_classification Virus-like particle Biochemistry Nucleic acid Lentinus Agronomy and Crop Science
Zdroj:	Annals of Applied Biology. 122:271-277
ISSN:	1744-7348 0003-4746
DOI:	10.1111/j.1744-7348.1993.tb04032.x
Popis:	Summary Isometric virus-like particles (VLPs) with diameter of approximately 34 nm containing ss-RNA were purified from abnormal mycelium of Shiitake mushroom, Lentinus edodes (Berk.) Sing. SDS-polyacrylamide gel elec-trophoresis demonstrated that the virions contain a single capsid polypeptide with molecular weight of about 22 000 daltons. The nucleic acid extract from purified VLPs preparations showed only one band with a size of approximately 7.3 kilobases. The susceptibility to RNase 1 and S1 digestions, resistance to DNase and thermal denaturation behaviour of the viral genome indicated that it is a single-stranded RNA. To our knowledge, isometric single-stranded RNA VLPs isolated from Shiitake mushroom mycelia have not been reported before.
Databáze:	OpenAIRE
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