RP-HPLC-DAD/MS/MS-based metabolic profiling of glycogen phosphorylase inhibitory active fractions of Nauclea latifolia stem bark
| Autor: | Sami A. Khalid, SO Abd Algaffar, HA Ali |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Pharmacology
Nauclea Chromatography biology DPPH Organic Chemistry Ethyl acetate Pharmaceutical Science biology.organism_classification Analytical Chemistry chemistry.chemical_compound Glycogen phosphorylase Complementary and alternative medicine chemistry Chlorogenic acid Glucoside Drug Discovery Molecular Medicine Quercetin Isorhamnetin |
| Zdroj: | Planta Medica. 79 |
| ISSN: | 1439-0221 0032-0943 |
| DOI: | 10.1055/s-0033-1352086 |
| Popis: | N. latifolia stem bark methanolic extract and the chloroform and ethyl acetate fractions significantly inhibited HLGP at a concentration of 5 mg/ml with 85%, 69% and 98%, respectively. On the other hand, i n vitro screening with 2,2-diphenyl-1-picrylhydrazyl (DPPH) at concentration of 12.5 ?g/ml revealed a prominent radical scavenging activity (RSA) of the chloroform fraction (85%) and relatively lower RSA of the methanolic (68%) and ethyl acetate fraction (77%) which seems not to be closely correlated with anti-diabetic activity. RP-HPLC-DAD coupled with tandem mass spectrometry performed on the ethyl acetate fraction which was associated with the most significant HLGP inhibitory activity resulted in the identification of chlorogenic acid, its glucoside, caffiec acid dimer, noengenin and noengenin dimmer, apigenin7- O-pentouronic acid, quercetin, luteolin 7-methoxy, isorhamnetin and isorhamnetin 3-methoxy |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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