Cyclic-nucleotide- and Ca2+/calmodulin-regulated channels in plants: targets for manipulating heavy-metal tolerance, and possible physiological roles

Autor:	Boaz Kaplan, Ramanjulu Sunkar, Tzahi Arazi, Hillel Fromm
Rok vydání:	2000
Předmět:	Transgene Heavy metals Genetically modified crops Biology Biochemistry Phenotype Cell biology Metal Cyclic nucleotide chemistry.chemical_compound Transmembrane domain chemistry visual_art Botany visual_art.visual_art_medium Ca2 calmodulin
Zdroj:	Biochemical Society Transactions. 28:471-475
ISSN:	1470-8752 0300-5127
Popis:	Recently we discovered a tobacco protein (designated NtCBP4) that modulates heavy-metal tolerance in transgenic plants. Structurally, NtCBP4 is similar to mammalian cyclic-nucleotide-gated non-selective cation channels containing six putative transmembrane domains, a predicted pore region, a conserved cyclic-nucleotide-binding domain, and a high-affinity calmodulin-binding site that coincides with its cyclic-nucleotide-binding domain. Transgenic tobacco expressing the plasma-membrane-localized NtCBP4 exhibit improved tolerance to Ni2+ and hypersensitivity to Pb2+, which are associated with a decreased accumulation of Ni2+ and an enhanced accumulation of Pb2+ respectively. Transgenic plants expressing a truncated version of NtCBP4, from which regulatory domains had been removed, have a different phenotype. Here we describe our approach to studying the involvement of NtCBP4 in heavy-metal tolerance and to elucidate its physiological role.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9c3bf9ea6cb3d9c0be8203430518d484 https://doi.org/10.1042/bst0280471 Zobrazit plný text záznamu