First complete genome sequence of an isolate of cowpea severe mosaic virus from South America
| Autor: | Lorhan L. Leal, F. Murilo Zerbini, Renan de Souza Cascardo, José Evando A. Beserra, Kelvin Josemar M. L. Teixeira |
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| Rok vydání: | 2021 |
| Předmět: |
Genetics
Whole genome sequencing Sanger sequencing 0303 health sciences 030306 microbiology Nucleic acid sequence General Medicine Biology Genome 03 medical and health sciences Open reading frame symbols.namesake Virology symbols ORFS Molecular Biology 030304 developmental biology Sequence (medicine) Genomic organization |
| Zdroj: | Virus Genes. 57:238-241 |
| ISSN: | 1572-994X 0920-8569 |
| DOI: | 10.1007/s11262-021-01831-2 |
| Popis: | In this study, the complete nucleotide sequence of a Brazilian isolate of cowpea severe mosaic virus (CPSMV) is presented for the first time. To date, the CPSMV-DG isolate, from the USA, is the only one with the complete known genome. High-throughput sequencing (Illumina HiSeq) and Sanger sequencing of the total RNA extract from a cowpea plant collected in Teresina city, Brazil, revealed the genome sequence of the CPSMV-Ter1 isolate. RNA-1 and RNA-2 are, respectively, 5921 and 3465 nucleotides (nt) long without the poly(A) tail, and show 77.91% and 76.08% nt sequence identity with CPSMV-DG, considered the type isolate of the species. The open reading frames (ORFs) were determined and the cleavage sites of the polyproteins were predicted. Although the two isolates show a similar genomic organization, there was a low percentage of sequence identity between Ter1 and DG. Furthermore, pairwise comparisons of a partial RNA-1 fragment between CPSMV-Ter1 and 11 CPSMV isolates from Brazil indicated 94.6 to 94.8% nt and 98.9% to 99.4% aa sequence identities. |
| Databáze: | OpenAIRE |
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