| Autor: |
Claude Bonne, J. Dornand, G. Modat |
| Rok vydání: |
1994 |
| Předmět: |
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| Zdroj: |
Oxidative Stress, Cell Activation and Viral Infection ISBN: 9783034874267 |
| DOI: |
10.1007/978-3-0348-7424-3_8 |
| Popis: |
We submitted cultured human umbilical vein endothelial cells to 5h hypoxia followed by 20 min to 24h reoxygenation. This resulted in an increased adhesion of human resting neutrophils to monolayers with two peaks after 20 min and 4h reoxygenation. This was correlated with the respective expression of the preformed granule membrane protein 140 (GMP-140) and of the de novo synthesized endothelial leukocyte adhesion molecule 1 (ELAM-1) on endothelial surface. We also found that the spontaneous endothelial production of interleukin 1 (IL-1) α and β, and interleukin 6 (IL-6) greatly increased after 5h hypoxia/19h reoxygenation. Superoxide dismutase, catalase or glutathione peroxidase added to culture before hypoxia efficiently prevented both effects. These results underline the crucial role played by endothelial-derived oxygen free radicals at reoxygenation in leukocyte adhesion and cytokine synthesis, which could lead to amplify the oxidative stress injury. The protection offered by free radical scavengers emphasizes the potential interest of antioxidants in post ischemic vascular disorders. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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