Three-dimensional structure of porcine pancreatic carboxypeptidase B with an acetate ion and two zinc atoms in the active site
| Autor: | V. Kh. Akparov, Nader Maghsoudi, Vladimir I. Timofeev, Inna P. Kuranova |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification 030102 biochemistry & molecular biology biology Resolution (mass spectrometry) Inorganic chemistry Active site chemistry.chemical_element General Chemistry Polyethylene glycol Zinc Condensed Matter Physics Zinc atom 03 medical and health sciences chemistry.chemical_compound Crystallography Pancreatic carboxypeptidase B 030104 developmental biology Enzyme chemistry biology.protein General Materials Science Acetate ion |
| Zdroj: | Crystallography Reports. 62:249-253 |
| ISSN: | 1562-689X 1063-7745 |
| DOI: | 10.1134/s106377451702002x |
| Popis: | Crystals of porcine pancreatic carboxypeptidase B (CPB) were grown by the capillary counter-diffusion method in the presence of polyethylene glycol and zinc acetate. The three-dimensional structure of CPB was determined at 1.40 A resolution using the X-ray diffraction data set collected from the crystals of the enzyme at the SPring 8 synchrotron facility and was refined to Rfact = 17.19%, Rfree = 19.78%. The structure contains five zinc atoms, two of which are present in the active site of the enzyme, and an acetate ion. The arrangement of an additional zinc atom in the active site and the acetate ion is different from that reported by Yoshimoto et al. |
| Databáze: | OpenAIRE |
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