| Popis: |
Aims: The aim of this study was In-Silico structural annotation of an amino acid sequence of Methylthioadenosine Nucleosidase Protein Zm00014a_031618 in Maize (Zea mays) retrieved from NCBI with the accession number PWZ58979. Study Design: The use of In-Silico studies for the structural annotation of Methylthioadenosine Nucleosidase protein. Place and Duration of Study: The research was conducted at the Bioinformatics Laboratory, Chevron Biotechnology Centre, Modibbo Adama University of Yola, Nigeria. Between June 2018 to July 2018. Methodology: The Methylthioadenosine Nucleosidase protein was retrieved from NCBI, physical and chemical parameters were calculated using ExPASy - ProtParam tool, the server SOPMA was used for secondary structure analysis (helix, sheets, and coils) and I-TASSER was used to obtain the 3D structure. Results: ExPASy - Prot Param tool computated the various physical and chemical parameters such as molecular weight (MW) 30117.97, total number of positively (+R) 27, negatively charged residues (-R) 30, theoretical isoelectric point (pI) 5.96, aliphatic index (AI) 103.67 and grand average hydropathy (GRAVY) 0.293. The SOPMA server was used for calculating the secondary structural features of protein sequences as Alpha helix 39.16%, Extended strand 14.69%, Beta turn 6.64% and Random coil 39.51%. I-Tasser was used for predicting the 3D structure where 2qttA from PDB was used as the template. Conclusion: This study helped in understanding the structural analysis of the Methylthioadenosine Nucleosidase Protein Zm00014a_031618 in maize (Z. mays). |
