External validation of recombinant antigens for serodiagnosis of machine operator's lung
| Autor: | Gabriel Reboux, Coralie Barrera, Sandrine Roussel, Isabel Warfolomeow, Bénédicte Rognon, Laurence Millon |
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| Rok vydání: | 2013 |
| Předmět: |
0303 health sciences
Lung biology business.industry Public Health Environmental and Occupational Health External validation medicine.disease biology.organism_classification 3. Good health law.invention Serology 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure 030228 respiratory system Antigen law Elisa test Immunology Recombinant DNA Medicine Mycobacterium immunogenum business Hypersensitivity pneumonitis 030304 developmental biology |
| Zdroj: | American Journal of Industrial Medicine. 57:195-201 |
| ISSN: | 0271-3586 |
| Popis: | Background Machine operator's lung (MOL) is a hypersensitivity pneumonitis the diagnosis of which is difficult. Our laboratory previously developed an ELISA test using recombinant antigens from Mycobacterium immunogenum isolated in French plant. The objective was to validate the previous ELISA results with ten new suspected cases from Germany. Methods Two serological analyses were performed: ELISA with the six recombinant antigens, and electrosyneresis with crude antigens of M. immunogenum and three other main species isolated from contaminated metalworking fluids. Results The two recombinant antigens acyl-CoA dehydrogenase and dihydrolipoyl dehydrogenase, combined together, and electrosyneresis are useful in making the diagnosis regardless of the clinical and radiological data. Finally 9 out of the 10 suspected cases were declared as MOL. Conclusions Despite the geographical distance, the crude and recombinant antigens produced to investigate the clustered French cases also proved to be useful in diagnosing the suspected cases in Germany. Am. J. Ind. Med. 57:195–201, 2014. © 2013 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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