Heterogeneity in phagocytic and oxidative burst activities of neutrophils and neonatal calves
| Autor: | Ian A. Gardner, Mahendra B. Kabbur, L. J. Allen, James S Cullor |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
Hematology medicine.diagnostic_test Phagocytosis Biology In vitro Pathology and Forensic Medicine Microbiology Respiratory burst Flow cytometry chemistry.chemical_compound chemistry Dichlorofluorescein Internal medicine medicine biology.protein Anatomy Bovine serum albumin Opsonin |
| Zdroj: | Comparative Haematology International. 7:30-36 |
| ISSN: | 1618-565X 0938-7714 |
| Popis: | In vitro experiments were conducted to determine the influence of source of neutrophils (individual calves) and calf age on phagocytic and oxidative burst activities of neutrophils isolated from 106 clinically healthy Holstein-Friesian calves. Calves were categorised by age into four groups: group A, 3–7 days; group B, 8–14 days; group C, 15–21 days or group D, 22–31 days. Neutrophils were isolated (purity >90%, viability >97%) from EDTA-anticoagulated blood. Aliquots of 2 x 106 neutrophils were incubated at 37°C for 30 min with fluorescein-labelledEscherichia coli 0111:134 J5,E. coli 0157:117 andSalmonella dublin, and with pooled bovine serum as the opsonin. Percentage phagocytosis and averaged number of intracellular bacteria per neutrophil were evaluated by fluorescence microscopy. Mean channel intensity was determined by flow cytometry using dichlorofluorescein diacetate and phorbol myristate acetate to evaluate 11202 production, a measure of oxidative burst. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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