Diferenciación morfofisiológica y molecular por curvas de melting de alta resolución (HRMA) y secuenciación del complejo Trichophyton mentagrophytes en cepas humanas en Valparaíso, Chile
| Autor: | Eduardo Álvarez Duarte, Peggy Vieille Oyarzo, Rodrigo Cruz Choappa |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
0303 health sciences biology 030306 microbiology General Medicine Trichophyton rubrum biology.organism_classification medicine.disease_cause Trichophyton schoenleinii Microbiology 03 medical and health sciences Trichophyton erinacei Species level Mycology Dermatophyte medicine Trichophyton Trichophyton benhamiae |
| Zdroj: | Revista Argentina de Microbiología. 52:88-95 |
| ISSN: | 0325-7541 |
| DOI: | 10.1016/j.ram.2019.05.002 |
| Popis: | Trichophyton mentagrophytes complex is the most frequent agent found in dermatophyte isolates after Trichophyton rubrum. It is divided into 3main groups: (1) Trichophyton benhamiae and related species; (2) Trichophyton simii and 2related species, Trichophyton quinckeanum and Trichophyton schoenleinii; and (3) T. mentagrophytes, T. interdigitale, and related species. They are all difficult to identify by morphophysiology. With the aim of classifying them at the species level, a descriptive study was performed on 17 isolated clinical strains identified in the Mycology Laboratory of the Universidad de Valparaiso as belonging to the T. mentagrophytes complex. They were compared with 3 reference strains (T. mentagrophytes CBS 318.56, T. interdigitale CBS 428.63, Trichophyton erinacei CBS 511.73). Morphophysiological and molecular tests were performed by high resolution melting analysis curves and ITS regions sequencing. Phenotypically, 3 species of the complex were identified. Molecular analyses reclassified all the species as belonging to T. interdigitale. In conclusion, no reliable morphophysiological patterns were established to differentiate between the species of the complex. Molecularly, all the strains studied were classified as T. interdigitale. |
| Databáze: | OpenAIRE |
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