Rapid detection of cardiac troponin I using antibody-immobilized gate-pulsed AlGaN/GaN high electron mobility transistor structures
| Autor: | Michael A. Mastro, Stephen J. Pearton, Soohwan Jang, Patrick H. Carey, Michael L. Good, Jiancheng Yang, Yu-Lin Wang, Fan Ren |
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| Rok vydání: | 2017 |
| Předmět: |
010302 applied physics
Materials science Physics and Astronomy (miscellaneous) business.industry Molecular biophysics Transistor 02 engineering and technology High-electron-mobility transistor Nitride 021001 nanoscience & nanotechnology 01 natural sciences Signal law.invention law Ionic strength 0103 physical sciences Electrode Troponin I Optoelectronics 0210 nano-technology business |
| Zdroj: | Applied Physics Letters. 111:202104 |
| ISSN: | 1077-3118 0003-6951 |
| Popis: | We report a comparison of two different approaches to detecting cardiac troponin I (cTnI) using antibody-functionalized AlGaN/GaN High Electron Mobility Transistors (HEMTs). If the solution containing the biomarker has high ionic strength, there can be difficulty in detection due to charge-screening effects. To overcome this, in the first approach, we used a recently developed method involving pulsed biases applied between a separate functionalized electrode and the gate of the HEMT. The resulting electrical double layer produces charge changes which are correlated with the concentration of the cTnI biomarker. The second approach fabricates the sensing area on a glass slide, and the pulsed gate signal is externally connected to the nitride HEMT. This produces a larger integrated change in charge and can be used over a broader range of concentrations without suffering from charge-screening effects. Both approaches can detect cTnI at levels down to 0.01 ng/ml. The glass slide approach is attractive for inexpensive cartridge-type sensors. |
| Databáze: | OpenAIRE |
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