Determination of the Antioxidant Capacity of Red Fruits by Miniaturized Spectrophotometry Assays
| Autor: | Gaëlle Catanante, Jean-Louis Marty, Francisco Eduardo Paiva Silva, Danilo Braga Ribeiro, Gilvanda Silva Nunes, Magda Márcia Becker |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Reactive oxygen species Antioxidant Chromatography ABTS medicine.diagnostic_test DPPH medicine.medical_treatment Radical Chemical structure 010401 analytical chemistry General Chemistry medicine.disease_cause 01 natural sciences 0104 chemical sciences chemistry.chemical_compound chemistry Spectrophotometry medicine Oxidative stress |
| Zdroj: | Journal of the Brazilian Chemical Society. |
| ISSN: | 0103-5053 |
| DOI: | 10.21577/0103-5053.20190003 |
| Popis: | Natural sources with high antioxidant capacity represent an interesting potential to prevent or minimize the oxidative stress that causes many chronic diseases. The antioxidant capacity of red fruits (strawberry and cherry) was evaluated by miniaturized spectrophotometric methods 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitroblue tetrazolium (NBT). ABTS and DPPH colorimetric methods are based on the ability of antioxidants to scavenge synthetic free radicals produced in vitro, which have a different chemical structure from the natural reactive oxygen species generated in the human body. In this respect, the NBT method stands up because it is based on O2•– scavenging which is generated in vitro by enzymatic reaction systems. The spectrophotometric assays adapted on microtiter plates allowed a rapid, inexpensive and simultaneous analysis. Larger amounts of lipophilic and hydrophilic antioxidants were obtained from strawberry fruit, which showed the lowest 50% signal inhibition concentration (IC50) values. The fruit analyzed showed promising sources of bioactive compounds with high antioxidant properties. |
| Databáze: | OpenAIRE |
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