Determination of Amino Acids in Corn Seed by Gas Chromatography–Mass Spectrometry
| Autor: | Paula Podea, Sevastiţa Muste, Ioan Has, Stăncuţa Scrob, Monica Culea, Sonia Suvar |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Alanine
chemistry.chemical_classification Chromatography Biochemistry (medical) Clinical Biochemistry Lysine Biochemistry Analytical Chemistry Amino acid chemistry.chemical_compound chemistry Aspartic acid Electrochemistry Proline Gas chromatography–mass spectrometry Trichloroacetic acid Spectroscopy Histidine |
| Zdroj: | Analytical Letters. 48:37-46 |
| ISSN: | 1532-236X 0003-2719 |
| DOI: | 10.1080/00032719.2014.930869 |
| Popis: | A gas chromatography–mass spectrometry method was developed for the determination of free amino acids from corn inbred lines flour. Amino acids were extracted using 6% trichloroacetic acid. The extracts were purified using an ion-exchange solid phase, derivatized as trifluoroacetic butyl esters, and analyzed. The 15N-methionine was used as the internal standard. The method was repeatable for most of the amino acids (coefficient of variation was in the order of 20%), and good accuracy and limits of detection were obtained. Among the free amino acids determined, the highest concentrations were obtained for aspartic acid, proline, gamma-aminobutyric acid, lysine, alanine, glutamic acid, and histidine. The study established the variation of the free amino acids within the inbred lines. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|