HPRT? mutant T cells in the peripheral blood and synovial tissue of patients with rheumatoid arthritis

Autor: Rose Goldstein, H C Birnboim, Jacob Karsh, J L Cannons
Rok vydání: 1998
Předmět:
Zdroj: Arthritis & Rheumatism. 41:1772-1782
ISSN: 1529-0131
0004-3591
DOI: 10.1002/1529-0131(199810)41:10<1772::aid-art9>3.0.co;2-c
Popis: Objective To investigate the frequency and characteristics of hprt- mutant T lymphocytes in the peripheral blood and synovium of rheumatoid arthritis (RA) patients compared with controls, and to correlate these findings with disease parameters. Methods An hprt- T cell assay was performed on blood and synovial samples from 93 RA patients, 8 osteoarthritis (OA) patients, and 19 control subjects. T cell clones were studied by flow cytometry and evaluated for fibronectin adhesion. Results RA patients showed a 5-fold increase in the frequency of mutant T cells in the peripheral blood compared with that in control peripheral blood, and a further 10-fold increase in the mutant T cell frequency in synovial tissue. In OA patients, the synovium also had a significantly higher frequency of hprt- mutant T cells compared with the peripheral blood, but at a lower level than in the rheumatoid synovium. RA peripheral blood mutant T cell clones displayed elevated fibronectin adhesion and β1 integrin expression, similar to that observed in the RA synovial T cell lines. Conclusion The origin of the mutated T cells in the peripheral blood of these patients appears to be the inflamed synovium of RA, and to a lesser extent, of OA, where the cells are exposed to a mitogenic and genotoxic environment.
Databáze: OpenAIRE
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