| Popis: |
The aim of the research was to develop a direct dot-immunoassay on a nitrocellulose membrane (NCM) using a conjugate based on colloidal gold for the qualitative determination of the presence of rabies virus antigen in pathological material. As the test samples, we used brain samples of various animal species, which were positive during the initial study by methods of fluorescent antibodies: foxes and mice. IFA and indirect ELISA carried out using diagnostic kits manufactured at the Federal Center for Toxicological, Radiation and Biological Safety. The materials present the results of primary laboratory tests of the test system for the indication of rabies antigen based on direct dotimmunoassay (DIA) 81 samples of pathological material, the brain of various animal species. It was shown that DIA has 100% specificity, and its optical signals correlate with the results of indirect ELISA. The proposed DIA method, in addition to independent application, can also serve as a basis for the design of test systems based on immunochemical analysis. |
