Generation of mutants of a strain producing the new antibiotic batumin by means of transposon mutagenesis

Autor:	Smirnov Vv, Churkina Ln, A. N. Kravets, V. I. Perepnikhatka
Rok vydání:	2000
Předmět:	Genetics Transposable element Plasmid Strain (chemistry) medicine.drug_class Mutant Antibiotics medicine Transposon mutagenesis Biology Applied Microbiology and Biotechnology Biochemistry Microbiology
Zdroj:	Applied Biochemistry and Microbiology. 36:262-265
ISSN:	1608-3024 0003-6838
DOI:	10.1007/bf02742577
Popis:	Various plasmids carrying transposon Tn5 were used to generate insertion mutants synthesizing batumin, a unique antibiotic with a selective antistaphylococcal effect. One of the plasmids used provided a sufficient yield of the clones in question. An analysis of over 7000 clones allowed us to select the mutant clones with increased and decreased levels of batumin synthesis and the mutants that lost the ability to synthesize this antibiotic.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9707fc14411f0a644c428a528323757c https://doi.org/10.1007/bf02742577 Zobrazit plný text záznamu Full text from SpringerLink